Micropropagation of Banana

In the in-vitro multiplication of banana (Musa spp.) cv. Robusta and Grand Nain were studied. In the culturing of banana most widely used species is Grand Naine banana species. Before harvesting the sword suckers were collected and were treated with anti-fungal agent and cultured on Murashige and Skoog basal medium supplemented in multiplication or rooting media with 6mg BAP [Benzyl Aminopurine], 0.5mg NNA [Nepthal Acetic Acid] and 0.5 mg IAA [Indole Acetic Acid]. Observations were recorded for four sub culturing. Evaluations were done by counting the number of new shoots, leaves, roots produced at each subculture. Under in vitro conditions shoot tips coming from different rhizomes behaved differently. On the average 25 plants were produced from each shoot tip after four sub culturing. The important aspect of in-vitro propagation of banana is the potential use of this technique in developing new varieties of banana with desired traits. In-vitro propagation technique can be used as a tool in breeding for improvement of a triploid and sterile crop like banana, since more number of clonal propagated material can be obtained by this method.

Ranna Chaudhary*1 and Ruby Patel2 Click here to download file

Short communication | Published online : 24-Feb-2021