Research Journal of Agricultural Sciences
An International Journal
In present study exopolysaccharide was produced from Cronobacter muytjensii ATCC 51329 (T) and purified by extraction followed by dialysis. The present exopolysaccharide was characterized as heteropolysaccharide in nature by RP-HPLC. HRMS analysis revealed that the peak at 9.8, 10.1, 13.8, 15.0, 15.9 were corresponds to Glucose, Galactose, Arabinose, Xylose, Rhamnose respectively. The partially purified exopolysaccharide obtained from cronobacter spp. showed 28.40% and 244.905±2.69% of water solubility index and water holding capacity respectively. The emulsifying activity for xylene was higher (63.955±0.45) followed by coconut oil (61.225±0.19). Exopolysaccharide has maximum antioxidant activity (100%) at 5 mg/ml with an EC50 value was 0.1 mg/ml. Exopolysaccharide showed significant antibacterial activity against E. coli, P. auroginosa, S. aureus and B. subtillus with MIC values 11.41 μg/mL, 16.98 μg/mL, 18.11 μg/mL, 13.16 μg/mL respectively. Anticancer activity of exopolysaccharide was studied by MTT assayat the highest tested concentration (1000 μg/mL). The inhibitory effects of exopolysaccharide increased to 91.6±6.5% and 91.5±5.8% against HepG-2 and HeLa cell lines respectively. Hemolytic activity showed that the EPS has negligible toxicity on human RBCs. The present properties specify the potential use of EPS as water holding, emulsifying, antibacterial, antioxidant, and anticancer agent.
Research Article | Published online : 17-May-2021