Production and Optimization of Alginate by Azotobacter species Isolated from Soil

Alginates are biocompatible, biodegradable, and safe biopolymers that are employed in a variety of pharmaceutical and medical applications as an anti-inflammatory, immobilization agents, radioactive suppression agents, and wound healing agents. In the present study, bacteria Azotobacter were isolated from rhizospheric soil, identification was carried out using selective media and biochemical methods. The screening for the production of alginate was carried out. The screened potential strain was checked for its ability to produce alginate using an alginate production assay. Characterization of the extracted compound was done by Fourier-Transform Infrared Spectroscopy (FTIR). Optimization of parameters like Carbon sources, Organic and Inorganic nitrogen sources, pH was carried out using Plackett Burman Design of Experiment, the production of alginate biopolymer from Azotobacter species was studied by statistical method and the optimization study concludes that Azotobacter (S1J) strain gives the highest production of alginate. In the present study the production of alginate biopolymer by optimizing medium from diazotrophic bacteria Azotobacter species successfully studied and found increased. The findings support commercial synthesis of alginate from bacterial sources, as it is of higher quality than algal alginate.

Snehalata Jadhav*1 and J. V. Kurhekar2 Click here to download file

Research Article | Published online : 23-Jun-2022