Research Journal of Agricultural Sciences
An International Journal
The objective of this study was to examine the in vitro wound healing effects of the ethanolic extracts of Ruellia tuberosa obtained by cytotoxicity, cell scratch assay, antioxidant activity, and selected phytochemical constituents. Qualitative phytochemical analysis and quantitative phytochemical analysis of Total phenolic and flavonoid contents were measured using spectrophotometry methods. The cytotoxic effects of the extracts on 3T3 cell line were evaluated by Mosmann, (1983) method. Furthermore, migration and spreading of the treated fibroblast cells were assessed by cell scratch assay as an in vitro wound healing model. The results of the cytotoxicity assay indicated that the ethanolic extract did not have any cytotoxic effect on fibroblast cells The cell was exposed to different concentrations of test compound for 48hrs and the cytotoxic effect of the extract was evaluated. The percentage viability 3T3 Cell line at the highest treated concentration of Ruellia tuberosa leaf extract was observed to be 77.97. Fibroblast migration was significantly increased by sample treatment compared to the control. The extracts showed good antioxidant activity and phytochemical compounds. The results showed that Ruellia tuberosa leaf extracts have wound healing potential and contain several important antioxidant phenolic compounds. This species deserves further investigation aiming to isolate and identify the active compounds.
Research Article | Published online : 13-Jul-2022