P- ISSN: 0976-1675
E- ISSN: 2249-4538

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Article


Protein Profiling and 3D Structure Prediction Studies on Mutated HCC-Associated Catenin Beta-1 Gene

Hepatocellular carcinoma (HCC) is considered to be the third leading cause of mortality due to cancer globally. Mutations in CTNNB1 (Catenin Beta-1) genes are considered to be responsible for the development of HCC with inconstant frequencies based on the etiology. The main objective of this in silico research work is to predict the 3D structure of the mutated protein sequence of the CTNNB1 (target) and to explore the positive electrostatic surfaces of the modelled protein structure. In this study, we use proteomics servers for sequence retrieval. We then explore the 3D structure of CTNNB1 using an automated homology modelling server which is then validated. Further, we identify the potential electrostatic regions of CTNNB1 and analyze them using advanced in silico tools. All the information will be predicted in 3D form using molecular visualization tools. Our results clearly elucidate the presence of positive electrostatic surfaces on the predicted 3D structure of the hepatocellular carcinoma gene-coded mutated protein, CTNNB1. The identified results play an indispensable role in the molecular interaction of drugs and proteins. Based on the results, we can validate the efficiency of the drugs and predict drugs using structure-based drug designing technique.

Research Article | Published online : 03-Aug-2022