P- ISSN: 0976-1675
E- ISSN: 2249-4538

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In-Vitro Evaluation of Antimicrobial and Antibiofilm Activity of Leaf Extract of Costus pictus D. Don against Multidrug Resistance Isolates

Antimicrobial resistance has become a serious public health risk with the increased use of antibiotics. Modern plant medicinal products can be a solution to this issue, since they are relatively safe and have additional beneficial effects. The aim of this study was to evaluate antimicrobial effect of Costus pictus D. Don solvent leaf extract. Antimicrobial effect of ethanol and chloroform extracts was tested by modified agar well diffusion method. Four types of bacterial isolates and 2 of fungal isolates were studied. After 24 hours inhibition period, zone of inhibition was measured. Furthermore, antibiofilm activity also carried out with plant extract by using titter plant well method. Among the 2 solvent extracts, ethanol showed good inhibitory activity against all isolates especially while using 7.5mg of extract. The antibiofilm report was confirmed that significant amount of biofilm production inhibited by plant extract. The major components found in the fractions were Cyclohexanone, N-Acetylmannosamine, Ethanamine, and 2-Furanone as analyzed by GC–MS/MS technique. It can be concluded that a high polar active compound present in the Costus pictus extract may be responsible for the significant antimicrobial activity.

Research Article | Published online : 04-Aug-2022