In vitro Antitumor Activity of Sargassum wightii Greville (Sargassaceae) Chloroform Extract against Human Cancer Cells

Seaweeds are exploited for their rich source of pharmacologically bioactive secondary metabolites and phytocompounds for anticancer properties. In the present study, the chloroform extract of Sargassum wightii was tested for antitumor activity against human liver, colon and breast cancer cells. MTT assay was adopted, and the percentage of cell viability was calculated. The inhibitory activity of Sargassum wightii chloroform extract tested at five different concentrations of treatments (25, 50, 100, 250 and 500µg/mL) with control against three different cancer cells showed significant inhibition at higher concentrations. The dose-dependent chloroform extract of Sargassum wightii inhibited cell growth inhibitions in vitro 85% of liver cancer cells (hepG2), 70% of colon cancer cells (HT-29), and 55% of breast cancer cells (MCF7) when compared with control. Cell morphology studies undertaken exhibited the presence of dead cancer cells of liver, colon and breast at the highest concentration (500µg/mL). Detailed examination are needed on the mechanisms involved in cell death, as Sargassum wightii chloroform extract was found to exhibit promising results towards anticancer property.

Vaithiyanathan Selvi*1, Subramanian Arivoli2 and Samuel Tennyson3 Click here to download file

Research Article | Published online : 27-Jan-2023