Optimization and Production of Nattokinase Enzyme from Endophytic Microorganisms

In the present study attention is focused on the microorganisms has potential source of compounds to produce Nattokinase enzyme. The objectives of this study, the microorganisms were screened and optimized for Nattokinase enzyme, production and purification of enzyme, hemolysis and fibrinolytic activity. Totally 3 bacteria such as Bacillus subtilis, B. licheniformis and B. megaterium and 2 fungi such as A. fumigatus and A. japonicus were screened from three different mangrove plant leaves like Avicennia marina, Salicornia bracheata and Suaeda maritima at Kattumavadi, Kottaipattinam and Sethubavasathiram – Tamil Nadu, India. The Nattokinase enzyme complex was screened from isolated endophytic bacteria and fungi by plate assay method. In screening, the maximum zone of clearance was recorded in Bacillus subtilis (25.0±2.5 mm) and A. japonicus (20.3±1.2 mm) than other strains. The optimization and production were analyzed from nattokinase enzyme using endophytic microorganisms. Partial purification of nattokinase enzyme was maximum from selected bacteria and fungi such as B. subtilis, B. licheniformis and A. japonicus were analyzed. The hemolysis, fibrinolytic activity, blood clot and lysis rate percentage were recorded. It was concluded that, these findings suggest that the nattokinase enzyme may act as excellent source in pharmaceutical industry. Characterization of the purified enzyme showed promising results for future studies and commercial production.

B. Megala*1 and P. Thirumalaivasan2 Click here to download file

Research Article | Published online : 28-Mar-2023