Siderophores are secondary metabolites camouflaged in the presence of low iron conditions, which may act as ferric iron chelating agents, and their potentiality to control phytopathogenic fungi as well as bacteria that study have been inspired in the modern centuries. Siderophores secreted by Pseudomonas species have been extensively used as biocontrol agents and it is a substitute to control phytopathogenic microorganism in the field of agriculture. In the present study, Pseudomonas fluorescens strain secreted extracellular siderophores when it has been grown in Kings’B medium under low iron conditions. The optimal medium composition for the secretion of siderophore was 10 μM iron concentration, 0.1% maltose and glycine concentrations, temperature 30oC, incubation time 72 hours, and pH 7.0. The siderophores produced under optimal conditions showed better antagonistic effects to fungal pathogens like Fusarium oxysporum and Sclerotium rolfsii. Hence Pseudomonas fluorescens strain was considered as an efficient biocontrol agent for fungal pathogens through siderophore metabolite secretion.
Research Article | Published online : 22-May-2023