Quantification of Active Phytoconstituents in Ethanolic Extract of Mentha piperita by Modern Analytical Tools

The present study investigates the qualitative and quantitative analysis of the bioactive constituents mostly preservative of medicinally important selected plant. The quantitative method for ethanolic extract of Mentha Piperita (Extract 3A) was developed using spectroscopic methods such as UV spectroscopy, IR spectroscopy, GCMS, also chromatographic methods such as TLC, HPTLC. The present active phytoconstituents in Mentha piperita that possess antimicrobial and antifungal activity are Menthol and Pulegone. The modern tools like gas chromatography and mass spectroscopy are excellently work to analyses phytoconstituents from natural herb extract. Using this technique, it is very easy to achieve separation of both active constituent with full m/z range. In GC method the experiment was done on thermofischer instrument using chromeleon software. The column DB-1 (25m×0.32mm×1.0µm) was used as stationary phase and mobile phase was helium is used with optimized flow rate. In GCMS method Stationary phase was Rxi-5ms, Restek (30 m x 0.25 mm x 0.25 μm) and mobile phase was helium with 1.4ml/minute flow rate. The validation key parameters were also performed. After quantification by modern analytical tool the Extract 3A shows presence of active phytochemical in ratio of 1:10 equivalency with that of standard drug.

Shital J. Patil*1, Khemchand R. Surana2 and Sunil K. Mahajan3 Click here to download file

Research Article | Published online : 03-May-2024