Anticoagulant and Antioxidant Activities of Water-soluble Polysaccharide from Fruiting Bodies of Hypsizygus ulmarius

This study was carried out to elucidate the anticoagulant and antioxidant activities of water-soluble alkali polysaccharide (HUAP) from Hypsizygus ulmarius mushroom. Antioxidant activities of HUAP was evaluated by DMPD, Hydroxyl radical scavenging activity, FRAP, Lipid peroxidation inhibition assay. Moreover, there in vitro anti-coagulant activities was evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) assays. In additionally HUAP having notable scavenging action against DMPD (EC50 = 13.29 mg/mL), Hydroxyl radicals (EC50 = 3.48 mg/mL), Lipid peroxidation inhibition (EC50 = 0.541 mg/mL) and also exhibited Ferric reducing power (FRAP) (EC50 = 1.97 mg/mL). At 5mg/ml, HUAP’s anticoagulant activity using APTT, PT and TT was calculated to be 42.15s, 14.2s and 25.25s, respectively. Antioxidant assay showed that HUAP exhibited strong radical scavenging activity, reducing power and LPO inhibition. HUAP had remarkably superior antioxidant activity and might be developed as a natural antioxidant. HUAP exhibited potent anticoagulant activity and would be expected to be a potential source of anticoagulant.

Aswini Moorthy1 and Sudha Govindan*2 Click here to download file

Research Article | Published online : 01-Aug-2024