P- ISSN: 0976-1675
E- ISSN: 2249-4538

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Phytochemical Screening, Thin layer Chromatographic Profiling and Antimicrobial Activities of Hedychium coronarium

The present study was aimed to preliminary phytochemical screening, thin layer chromatographic profiling and antimicrobial activities of Hedychium coronarium. Phytochemical screening was performed to investigate bioactive constituents of dried flowers by utilizing various solvent system (petroleum ether, dichloromethane, ethyl acetate, methanol and water) to confirm the presence of alkaloid, flavonoids, saponins and glycosides etc. Total phenol and flavonoid content were examined spectrophotometrically. The crude extracts (dichloromethane, ethyl acetate, methanol and aqueous) which shows good phytochemical result was subjected to TLC profiling and separation of spots were observed under normal day light, shorter wavelength (254nm) and longer wavelength (365nm) of light by using different solvent systems with Rf values compared to standard drugs of gallic acid (0.28) and quercetin (0.50). The antimicrobial activity was determined by well diffusion method along with standard antibiotic against selected bacterial and fungal strains. In preliminary phytochemical screening methanol and aqueous extracts of flower contain higher bioactive constituents than other solvent extract. Total phenol and flavonoid contents were ranged from (0 to 1.57±0.01) mg GAE/g and (1.76±0.02) to (4.13±0.01) mg QE/g. Among all the solvent extracts, dichloromethane, methanol and aqueous extracts showed well separation of spots. The retardation factor was found to be 0.22 and 0.50 to 0.62 which is similar to standard gallic acid and quercetin respectively. The methanol extract demonstrated effective antimicrobial activity against the tested pathogenic species with minimum inhibitory concentrations values ranging from 25-100 mg/mL for bacteria and fungi respectively. Methanolic extract of Hedychium coronarium flower exhibit the presence of various phytoconstituents and display significant antimicrobial activity.

Research Article | Published online : 25-Oct-2024