P- ISSN: 0976-1675
E- ISSN: 2249-4538

© CARAS (Centre for Advanced Research in Agricultural Sciences)
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Volume- 17 - March-April 2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 121–139
Ajaz A. Shah*1
1 Department of Agriculture Production and Farmers Welfare, Kashmir Division, Government of Jammu and Kashmir, India
Abstract
Beekeeping, one of the world’s oldest apiculture practices, has transformed from ancient honey hunting into a globally significant agricultural and ecological enterprise. Historical records and archaeological evidence highlight its cultural, nutritional, and medicinal importance across civilizations, while the introduction of movable-frame hives in the 19th century marked a pivotal shift toward scientific and sustainable practices. Today, apiculture combines traditional knowledge with modern technologies such as migratory beekeeping, selective breeding, integrated pest management, and artificial intelligence-based hive monitoring to improve colony health, productivity, and pollination efficiency. Globally, annual honey production surpasses 1.8 million metric tonnes, with China, Turkey, Iran, India, and Argentina leading in output, and the United States and European Union emerging as major consumers and importers. Beyond honey, hive products including beeswax, royal jelly, pollen, propolis, and bee venom generate substantial economic value across food, pharmaceutical, cosmetic, and nutraceutical industries, contributing to the diversification of rural incomes and the expansion of high-value markets. Regionally, beekeeping supports poverty reduction, women’s empowerment, and export economies in Asia and Africa, while Europe emphasizes pollinator protection policies and the Americas focus on large-scale pollination services vital for commercial agriculture. Ecologically, honey bees function as keystone pollinators, ensuring biodiversity conservation, crop productivity, and ecosystem resilience, thereby reinforcing food and nutritional security. Economically, beekeeping is highly efficient, offering multiple revenue streams with low land and capital requirements, making it accessible to smallholders, landless farmers, and even urban communities. However, the sector faces persistent challenges, including colony losses due to pests and diseases, pesticide exposure, climate variability, habitat degradation, adulteration of honey, and inadequate institutional support. Addressing these constraints requires integrated approaches encompassing pollinator-friendly landscapes, adaptive beekeeping practices, product quality assurance, value addition, cooperative marketing, and enabling policy frameworks. Overall, beekeeping stands at the nexus of ecology, economy, and culture, representing a resilient, inclusive, and future-ready enterprise. Its potential to enhance sustainable agriculture, strengthen rural development, support biodiversity conservation, and contribute to climate change adaptation positions apiculture as a vital component of global sustainability strategies.

Research Article | Published online : 02-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 140–145
Imoru A.*1, Awoneye O. O.2, Oluwasegun O. B.3, Dickson F. B.4 and Omosule O.5
1-5 Department of Animal Production, Rufus Giwa Polytechnic, Owo, Ondo Sate, Nigeria
Abstract
This study examined the effects of dietary turmeric rhizome powder (TRP) supplementation on the external egg quality traits obtained from laying hens. The experiment was conducted at the Poultry Unit of the Teaching and Research Farm, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria. A total of 192 laying hens (16 weeks old) were randomly allocated to three dietary treatments, each comprising 64 birds. The treatments were further sub-divided into four replicates, with each containing 16 birds. The treatments included a control diet without turmeric (T1), a diet supplemented with 5 g/kg TRP (T2), and a diet supplemented with 10 g/kg TRP (T3). Key parameters assessed included egg weight, shell thickness, shell weight, egg width, egg height, and total egg production. Results indicated a significant difference (p<0.05) in total egg production among treatments, with the highest production observed in T2 (1704 eggs), followed by T1 (1660 eggs), and the lowest in T3 (1584 eggs). This suggests that moderate TRP supplementation enhances laying performance, whereas excessive inclusion may induce metabolic constraints. Eggshell thickness was significantly higher (p<0.05) in T3 (0.68 mm) compared to T2 (0.57 mm) and T1 (0.53 mm), indicating improved calcium deposition and enhanced shell integrity at higher TRP levels. Eggshell weight also varied significantly (p<0.05), with both T1 and T3 exhibiting the highest values (5.8 g), while T2 had the lowest (5.4 g). Egg weight remained statistically unaffected across treatments (p>0.05), with mean values of 56.93 g (T1), 53.70 g (T2), and 57.70 g (T3), suggesting that TRP supplementation did not significantly influence overall egg mass. Regarding storage stability, weight depreciation was significantly lower (p<0.05) in T3 (54.10 g to 53.68 g) compared to T1 (56.96 g to 54.96 g) and T2 (54.91 g to 52.99 g), reinforcing the role of TRP in maintaining egg mass and reducing moisture loss during storage. The findings suggest that dietary supplementation with 5 g/kg TRP optimizes egg production, whereas 10 g/kg enhances eggshell integrity and storage stability. However, the observed decline in egg production at the higher supplementation level indicates a possible threshold beyond which TRP may negatively impact laying performance. Further research is required to elucidate the physiological mechanisms underlying these effects and to determine the optimal TRP inclusion level for maximizing both egg production and external egg quality traits.

Research Article | Published online : 05-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 146–152
S. R. Reshma1, M. T. Ranjith*1, Mani Chellappan1, E. R. Harish2, C. V. Vidya1 and A. G. Kiran3
1 Department of Agricultural Entomology, College of Agriculture, Vellanikkara, KAU, P. O., Thrissur - 680 656, Kerala, India 2 Division of Crop Protection, ICAR- Central Tuber Crops Research Institute, Sreekaryam, Thiruvananthapuram, Kerala India 3 Centre for Plant Biotechnology and Molecular Biology, College of Agriculture, Vellanikkara, KAU, P. O., Thrissur - 680 656, Kerala, India
Abstract
The tobacco whitefly, Bemisia tabaci, is a major global pest, and its complex nature poses significant challenges to agriculture, particularly in its role as a vector for severe plant viruses like Brinjal yellow mosaic virus (BYMV). In Kerala, India, this pest severely impacts brinjal (Solanum melongena) cultivation, an economically vital crop. Recognizing the pest's reported high morphological plasticity and the existence of cryptic species, this study aimed to characterize the intraspecific morphological variability of Bemisia tabaci pupae collected from brinjal plants across seven diverse agro-ecological units (AEUs) in Kerala. Using standard clearing, staining, and mounting techniques, fifteen key puparial morphometric traits were measured and analyzed across fourteen districts of Kerala. While initial observations showed subtle differences in overall dimensions, Principal Component Analysis (PCA) effectively revealed significant population segregation. The first two principal components (PC1 and PC2) collectively explained 75.3% of the total variance. PC1 was strongly driven by pupal width (PW), operculum length (OL), and lingula length (LL), clearly separating populations of Wayanad and Ernakulam as having distinct, larger pupal size-related features. PC2, on the other hand, was influenced by wax margin dimensions (LRWM, LLWM) and vasiform orifice length (VOL), highlighting populations Kottayam, Idukki and Palakkad. These results confirm the existence of distinct morphotypes within Bemisia tabaci infesting brinjal in Kerala, suggesting that local environmental conditions, host plant factors, and micro-climatic variation are driving phenotypic differentiation. This structural diversity emphasizes the pest's high adaptability, underscoring the necessity for location-specific and integrated pest management strategies.

Research Article | Published online : 09-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 153–157
Aisha Parween*1 and Vidyanath Jha2
1 University Department of Biotechnology, Lalit Narayan Mithila University, Kameshwaranagar, Darbhanga - 846 008, Bihar, India 2 Retired Professor of Botany, Lalit Narayan Mithila University, Kameshwaranagar Darbhanga, Bihar, India, Former Principal, M. R. M. College, Lalbagh Darbhanga - 846 004, Bihar, India
Abstract
Hordeum vulgare L. (barley) is one of the earliest domesticated cereal crops and has long been revered in India as a sacred and health-promoting grain. Despite its historical and cultural importance, barley cultivation declined during the Green Revolution era due to the expansion of high-yielding wheat varieties and changing dietary preferences. In recent decades, production has stabilized largely because of demand from the malt industry; however, renewed scientific evidence highlighting its nutritional and therapeutic benefits has repositioned barley as a promising functional food crop. This review compiles the present status of barley research in India with special emphasis on its potential in the food sector and outlines future prospects for its promotion as a health cereal. Barley is particularly valued for its high content of soluble dietary fibre, especially mixed-linkage β-glucans, which have been clinically associated with reduced serum cholesterol, improved glycemic response, and enhanced colon health. In addition to β-glucans, barley contains significant levels of amylose, arabinoxylans, essential minerals (Zn, Fe, Se), and diverse phytochemicals including phenolic acids, flavonoids, anthocyanins, and vitamin E compounds, contributing to strong antioxidant activity. Comparative analyses indicate that barley exhibits higher total phenolic content and antioxidant potential than several commonly consumed cereals, supporting its role in prevention of cardiovascular diseases, type 2 diabetes, and certain cancers. The review also highlights key physical and biochemical traits important for food barley improvement, including hulless grain types for enhanced processing efficiency, high β-glucan and amylose content for lower glycemic index, improved mineral biofortification, and enhanced disease resistance. Market analysis suggests a rapidly growing breakfast cereal sector in India, dominated by oats largely imported despite barley being an indigenous, climate-resilient crop with comparable health benefits. The limited development of palatable barley-based products and insufficient consumer awareness have constrained its market penetration. Potential value-added products such as multigrain atta, biscuits, bread, flakes, and ready-to-drink sattu offer significant opportunities for industry engagement. Strategic interventions including breeding high-yielding hulless varieties, product standardization, nutritional branding, awareness campaigns, and supportive pricing policies could revitalize food barley cultivation. With coordinated efforts among researchers, policymakers, and the food industry, barley has strong potential to enhance farmer income, strengthen nutritional security, and re-establish its position as a sustainable health cereal in India.

Research Review | Published online : 13-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 158–162
S. B. Fasale1, M. B. Waghmare2, P. R. Lawand3 and S. S. Khot*4
1 Department of Botany, Shri Shiv-Shahu Mahavidyalaya, Sarud - 416 214, Maharashtra, India 2 Department of Botany, The New College, Kolhapur - 416 012, Maharashtra, India 3 Department of Botany, Dahiwadi College, Dahiwadi - 415 508, Maharashtra, India 4 Department of Botany, Yashwantrao Chavan Warana Mahavidyalaya, Warananagar, Kolhapur - 416 113, Maharashtra, India
Abstract
The current study focuses on the characterization of Anisomeles indica (L.) Kuntze stem, a medicinal plant native to the Western Ghats, using Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX) and Fourier Transform Infrared Spectroscopy (FT-IR). SEM-EDX provided insights into the surface morphology and elemental composition of the stem. The EDX was employed to analyze the mass percentage, while SEM showed the presence of different elements viz., carbon, oxygen, sodium, magnesium, aluminum, silicon, phosphorus, chlorine, calcium, magnesium, and iron. The carbon was the highest component (45.95%) as compared with other minerals. FT-IR analysis was performed to identify peak values corresponding to various functional groups, including hydroxyl, alcohols, phenols, alkyl halides, alkanes, proteins, carboxylic acids, and lipids. The findings revealed the presence of essential phytochemicals and trace elements, confirming the therapeutic potential of Anisomeles indica. This study enhances the understanding of its structural and chemical properties, supporting its traditional medicinal applications and future pharmacological research.

Research Article | Published online : 16-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 163–169
R. Senthamil*1, Imtiyaz Qayoom1, J. Siva Shanmugam2, Vedant V. Kharabe3, Shailesh Prajapati3, P. R. Sidam4, Vishal S.1, Narendra S. B.5, V. B. Thorat6, Hemvarsha6 and R. B. Chamle7
1 Division of Aquatic Environmental Management, Faculty of Fisheries, SKUAST-Kashmir Rangil, Ganderbal, Kashmir, India 2 TNJFU- Dr. M. G. R. Fisheries College and Research Institute, Thalainayeru, Nagapattinam, Tamil Nadu, India 3 Division of Aquaculture, Faculty of Fisheries, SKUAST-K Rangil, Ganderbal, Kashmir, India 4 Department of Fisheries Resources, Economic, Statistics and Extension Education, College of fisheries Ratnagiri, Maharashtra 5 Division of Aquatic Animal Health Management, Faculty of Fisheries, SKUAST-K Rangil, Ganderbal, Kashmir, India 6 Department of Aquatic Animal Health Management, College of Fisheries, Hisar, CCS HAU Hisar, Haryana, India 7 Department of Fisheries Resources Management, College of Fisheries Hisar, CCS HAU Hisar, Haryana, India
Abstract
A research study was conducted to investigate the occurrence, prevalence, mean intensity and abundance of parasite infestations in the Cauvery Delta region during a span of 15-20 days. The study focused on cultured food and ornamental fishes, with a total of 30 different fish samples including carps, Nile tilapia and goldfish collected and brought to the laboratory for analysis. The fishes were examined for ectoparasites using a wet mount preparations under a light microscope, focussing on the gills, skin mucus, and fins like pelvic fins, pectoral and caudal fins. The examination revealed the occurrence of three ectoparasites: Argulus sp., Lernaea sp., and Trichodina sp. The overall parasite prevalence was recorded at 78.3%, with a mean intensity of 400 and an abundance of 0.78. Infection rates were significantly higher in larger fish, with some cases showing 100% prevalence, indicating a strong correlation between fish size and susceptibility to parasitic infections. The study also highlighted the influence of environmental factors such as water quality and stocking density on the severity of infestations. These findings emphasize the importance of routine health monitoring and effective management practices to control ectoparasitic infections and improve fish health in aquaculture systems.

Research Article | Published online : 19-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 170–177
Shabir Mir*1
1 Division of Animal Genetics and Breeding, FVSc & AH Shuhama, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar - 190 006, Jammu and Kashmir, India
Abstract
Salmonella enterica serovar Typhimurium is a major avian pathogen that causes severe intestinal damage in young chicken leading to significant economic losses in the poultry industry. This study was conducted to examine the temporal expression of chemokine CXCLi2 gene in Kashmir Commercial Layer (KCL) breed of chicken following experimental infection with Salmonella enterica serovar Typhimurium. Sampling was conducted at 0, 1, 3, 5, 7, 9, 11, 13 and 15 days post infection (DPI). Quantitative real-time reverse transcription PCR (qRT-PCR) was employed to assess mRNA expression levels in the liver, spleen and caecum. This experimental analysis revealed a significant upregulation of CXCLi2 expression in infected birds relative to age matched uninfected controls. Tissue specific kinetics indicated a peak in gene expression until 5 DPI in liver and until 7 DPI in spleen and caecum followed by a marked decline through 15 DPI. Among the tissues examined caecum exhibited the highest expression followed by the spleen and liver. Correspondingly leukocyte counts demonstrated a progressive increase during the first week post infection which then gradually declined over the subsequent period. These findings highlight the tissue specific and time dependent nature of early immune response in KCL chickens to Salmonella Typhimurium infection and suggest that CXCLi2 plays a critical role in orchestrating initial host defence.

Research Article | Published online : 23-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 178–180
Rahul Krishna B. L.*1, Mani Chellappan2, Smitha M. S.3, Ranjith M. T.4, Kiran A. G.5 and Jobiraj T.6
1-4 Department of Agricultural Entomology, College of Agriculture, Vellanikkara, Kerala Agricultural University, Vellanikkara, Thrissur - 680 656, Kerala, India 5 Centre for Plant Biotechnology and Molecular Biology, College of Agriculture, Vellanikkara, Kerala Agricultural University, Vellanikkara, Thrissur - 680 656, Kerala, India 6 Department of Zoology, Government Arts and Science College (Calicut University), Kozhikode - 673 018, Kerala, India
Abstract
The diversity of the sub-family Halictinae Thomson (Hymenoptera: Halictidae) in Kerala is represented by the genus Halictus Latreille, Lasioglossum Curtis, and Sphecodes Latreille. The study found the genus Seladonia grouped under the same taxon in Wayanad district of Kerala. Knowledge of the habitat and soil characteristics of their nest site is requisite for habitat-based conservation of these ground-nesting fauna. So, a brief investigation was carried out to understand their ecosystems, with special emphasis on the characteristics of the soils they nest in. The texture and aggregate strength of soil at the nesting site were analysed by the International Pipette method and Wet analysis, respectively. The ecosystem of the bees was usually undisturbed lands with hard lateritic soils. Through soil characterisation, the bees were found to inhabit clay loam with a very high aggregate strength of 6.1 mm mean weight diameter. The nest structure was studied by observing the excavated nest cast. Their subterranean nest directly opens to the ground surface and comprises 0.4 cm wide tunnels that end in 0.6 cm wide cells.

Research Article | Published online : 29-Mar-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 181–189
Lovepreet Singh1, Sandeep Kumar Singh*2 and Rajneesh Thakur3
1-3 Department of Agriculture, Mata Gujri College, Fatehgarh Sahib - 140 406, Punjab, India
Abstract
An investigation was carried out on “Effect of different levels of organic manures and munch materials on garlic (Allium sativum L.) under Fatehgarh Sahib condition” was conducted for two consecutive years (2018-19 and 2019-20) at Experimental Research Farm, Kharora, Department of Agriculture, Mata Gujri College, Fatehgarh Sahib, Punjab, India. The experiment comprised of sixteen treatments involving with four levels of factor O (Organic manures) viz., O0: No manure @ 0 t/ha, O1: Farm yard manure (FYM) @ 20 t/ha, O2: Vermicompost @ 5 t/ha, O3: Poultry manure @ 10 t/ha and four levels of factor M (Mulch materials) viz., M0: No mulch @ 0 t/ha, M1: Paddy straw @ 6 t/ha, M2: Saw dust @ 10 t/ha, M3: Rice husk @ 5 t/ha. The experiment was laid out in factorial randomized block design with three replications. The treatment consisted of O0M0: No Manure @ 0 t/ha + No Mulch @ 0 t/ha, O0M1: No Manure @ 0 t/ha + Paddy Straw @ 6 t/ha, O0M2: No Manure @ 0 t/ha + Saw Dust @ 10 t/ha, O0M3: No Manure @ 0 t/ha + Rice Husk @ 5 t/ha, O1M0: FYM @ 20 t/ha + No Mulch @ 0 t/ha, O1M1: FYM @ 20 t/ha + Paddy Straw @ 6 t/ha, O1M2: FYM @ 20 t/ha + Saw Dust @ 10 t/ha, O1M3: FYM @ 20 t/ha + Rice Husk @ 5 t/ha, O2M0: Vermicompost @ 5 t/ha + No Mulch @ 0 t/ha, O2M1: Vermicompost @ 5 t/ha + Paddy Straw @ 6 t/ha, O2M2: Vermicompost @ 5 t/ha + Saw Dust @ 10 t/ha, O2M3: Vermicompost @ 5 t/ha + Rice Husk @ 5 t/ha, O3M0: Poultry Manure @ 10 t/ha + No Mulch @ 0 t/ha, O3M1: Poultry Manure @ 10 t/ha + Paddy Straw @ 6 t/ha, O3M2: Poultry Manure @ 10 t/ha + Saw Dust @ 10 t/ha, O3M3: Poultry Manure @ 10 t/ha + Rice Husk @ 5 t/ha. Pooled data over two years revealed that maximum plant height (68.56 cm), number of leaves per plant (8.41), leaf length (60.18 cm), neck thickness (4.34 cm), number of cloves per bulb (28.19), clove length (4.05 cm), clove width (1.41 cm), average weight of bulb (40.01 g), average weight of clove (3.04 g), marketable yield per hectare (155.83 q), total yield per hectare (390.97 q) were recorded with the application of vermicompost @ 5 t/ha + Paddy Straw @ 6 t/ha. In terms of economics revealed that the application of vermicompost @ 5 t/ha + Paddy Straw @ 6 t/ha produced the highest gross returns of Rs. 935000.00, highest net income of Rs. 778783.39 and highest benefit cost ratio (4.99).

Research Article | Published online : 06-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 190–194
Divya Katiyar1, Kalyan Ghadei2, Nitin Rajput3, Akash Singh4 and Arjun Prasad Verma*5
1 Research Scholar, 2 Professor, Department of Extension Education, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi - 221 005, Uttar Pradesh, India 3-4 Research Scholar, Department of Agricultural Extension and Communication, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Gujarat, India 5 Assistant Professor, Department of Agricultural Extension, CoA, Banda University of Agriculture and Technology, Banda - 210 001, Uttar Pradesh, India
Abstract
The study aimed to know the consumption pattern of millets among the households in Banda, Kanpur and Lucknow districts of the Uttar Pradesh. Since an increasing number of people started to learn that millets are hardy against the tough weather and are full of nutrients, this study examined how they find their way to our daily meals. The research design was explanatory and total 150 respondents were chosen using random sampling. The findings showed that the consumption of millet was very high as 96.70 percent of the respondents indicated that they consumed the millets in different ways. Flour was the most desirable type in terms of its flexibility to the traditional cooking habits. It is interesting to note that 41.37 per cent of the respondents had started taking millets in the last one year which means that the diet has changed recently due to awareness and marketing campaigns. The study indicates that we need to have clear awareness campaigns and policies to ensure more people consume millets so that we can have better nutrition and create sustainable food systems that are sustainable and long-term.

Research Article | Published online : 08-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 195–199
H. D. Bhartiya*1
1 Mycopathology Laboratory, Department of Botany, Bipin Bihari P/G College, Jhansi - 284 001, Uttar Pradesh, India
Abstract
During 2020-2021 occurrence of new records of Cercosporoid fungi causing leaf spots disease on wild plants of North-Eastern Uttar Pradesh and Burdwan district of West Bengal. The typical disease symptoms were observed on both surface of leaves. Disease spots dry, necrotic, dark olivaceous brown in colour. On the basis of morphological and microscopic characteristics of the fungus Cercospora sp. were found to be associated with the leaf spots disease. These Cercosporoid fungi have been described and illustrated, viz. Cercospora phyllanthicola; C. ricinella, C. ternateae and C. traversiana on Phyllanthus indicus L. (Euphorbiaceae), Ricinus communis L. (Euphorbiaceae), Clitoria ternate L. (Fabaceae) and Trigonella coeruleae L. (Fabaceae) respectively. The specimens were collected in different parts of Gorakhpur District (UP) and Vardwan (WB). Photographs and the line drawing of the new records from India have been provided.

Research Article | Published online : 11-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 200–207
Tripti Sharma*1, Astha Gupta2 and Dolly Wattal Dhar1
1 Sharda School of Agricultural Sciences, Sharda University, Greater Noida - 201 310, Uttar Pradesh, India 2 Department of Biotechnology, Sharda School of Biosciences and Technology, Sharda University, Greater Noida - 201 310, Uttar Pradesh, India
Abstract
Microgreens are pivotal for human health as these are rich in useful components which can be controlled by growing conditions (light, substrate, temperature, genetic composition and stage of crop harvest). Amongst these, intensity, quality and duration of light are key determining factors. The aim of the present study was to evaluate impact of Light Emitting Diodes providing 96% Photosynthetically Active Radiation on growth attributes, photosynthetic pigments and sensory parameters amongst chickpea genotypes cultivated as microgreens till 28th day. A consistent enhancement was depicted in shoot height, number of leaves, fresh and dry weight on per plant basis and the photosynthetic pigments. The genotype Pusa-3022 exhibited better performance for all growth parameters. The genotype Pusa-4005 showed higher total pigments and ratio of chlorophyll a to b, while, the proportion of total chlorophyll to carotenoids was maximum in Pusa-3062. The performance of sensory parameters was greatest on 14th day. Correlation data depicted inconsistent observations amongst some parameters. In view of the results obtained, chickpea microgreens can be considered as a potential functional food supplement till 14th day of cultivation.

Research Article | Published online : 14-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 208–212
Parwez Qureshi*1, Sayeda Sameena2, Quadri S. Amreen3, Shinde Manisha Vitthal4 and Isra Bano Shaikh5
1-2 Assistant Professor, Department of Microbiology, A.E.V.P.M. Women’s College (Affiliated with SNDT University Mumbai), Aurangabad - 431 003, Maharashtra, India 3 Head of Home Science Department, A.E.V.P.M. Women's College of BCA, Chatrapati Sambhajinagar - 431 006, Maharashtra, India 4 Head of Department, BSc Cls, A.E.V.P.M, Women’s College of BCA, Chatrapati Sambhajinagar - 431 006, Maharashtra, India 5 Head of Science and Technology Department, A.E.V.P.M. Women's College of BCA, CIDCO Chatrapati Sambhajinagar - 431 006 Maharashtra, India
Abstract
Human Papillomavirus (HPV) is a major etiological agent of cervical cancer, particularly high-risk (HR) genotypes such as HPV 16 and 18. This study aimed to detect and genotype HR-HPV in cervical samples using real-time polymerase chain reaction (RT-PCR). Cervical swab samples were collected and subjected to DNA extraction followed by amplification targeting the E6/E7 oncogenic regions of 14 HR-HPV genotypes. The assay utilized fluorescence-based detection for qualitative analysis. Among the analyzed samples, only one case (age group 60-70 years) tested positive for HR-HPV, specifically genotype 39/68. No positive cases were observed in younger age groups. The findings highlight the presence of HR-HPV in older age groups and emphasize the importance of molecular screening for early detection. The study underscores the utility of RT-PCR as a sensitive diagnostic tool and reinforces the need for vaccination and preventive strategies to reduce HPV-associated cervical cancer risk.

Research Article | Published online : 20-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 213–218
Patale S. W.*1
1 Department of Botany, Swami Muktanand College of Science, Yeola - 423 401, Maharashtra, India
Abstract
Sustainable intensification of agriculture increasingly depends on biological processes that enhance soil health and nutrient-use efficiency. Arbuscular mycorrhizal fungi (AMF) constitute one of the most important functional groups of soil microorganisms, playing a vital role in nutrient acquisition, soil aggregation, and plant tolerance to environmental stresses. The present study evaluates the contribution of AMF to soil fertility and crop productivity in representative agricultural fields of Nashik District, Maharashtra, India. Ten fields under major crops onion, maize, wheat, soybean, and grape were assessed for AMF root colonization, spore density, soil physicochemical properties, and crop performance. Fields exhibiting higher AMF colonization showed significantly greater soil organic carbon, available nitrogen, phosphorus, and potassium, along with enhanced crop growth and yield. Yield improvement ranged from 12-20% across crops, with onion and soybean showing the highest responses. Strong positive correlations between AMF abundance and soil fertility indices confirm the central role of mycorrhizal symbiosis in sustainable nutrient management. The findings establish AMF as effective biological inputs capable of reducing dependency on chemical fertilizers while improving soil resilience. Integration of AMF into conventional and integrated farming systems is recommended to support climate-resilient and sustainable agriculture in semi-arid regions of India.

Research Article | Published online : 22-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 219–227
B. Tanuja Priya*1-3, B. N. S. Murthy2, Anand C. Reddy2 and C. Gopalakrishnan2
1 Department of Fruit Science, College of Horticulture, University of Horticultural Science, Bengaluru - 560 065, Karnataka, India 2 Division of Fruit Science, Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake Post, Bengaluru, Karnataka, India 3 Dr. Y. S. R. Horticultural University, Venkataramannagudem, West Godavari District - 534 101, Andhra Pradesh, India
Abstract
Bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae has become a serious threat in Southern Asian countries. The prime step in the management of this disease is the characterization of the pathogen. Thus, isolation and identification of pathogen through various methods was performed. Further, Xap strain was studied for evolutionary relatedness by comparing with various Xap strains across the countries representing various geographical origins deposited in National Center for Biotechnology Information (NCBI) Genbank, targeting 16 rRNA loci. It is worth mentioning that the Xap strain IIHR-1 from Indian Institute of Horticulture Research, Bengaluru, India showed a close proximity with strain MS41287 of Himachal Pradesh, India by sharing 98% identity. Thus, 16S rRNA gene aid to unravel the evolutionary relatedness of the Xap strains across the countries.

Research Article | Published online : 24-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 228–236
Trupti Vhanamane1, Priyanka Mashale1, Malika Nadaf2, Sadaf Deshmukh2 and Parashuram Patroti*2
1 School of life Sciences, Punyashlok Ahilyadevi Holkar Solapur University, Kegaon, Solapur - 413 255, Maharashtra, India 2 ICAR - Indian Institute of Millets Research, Regional Station, Centre on Rabi Sorghum, Shelgi, Solapur - 413 006, Maharashtra, India
Abstract
The present investigation was undertaken to assess combining ability, heterosis, and heterotic grouping for grain yield and its component traits in sorghum using a diallel mating design (excluding reciprocals). The analysis of variance revealed highly significant differences among genotypes, with both general combining ability (GCA) and specific combining ability (SCA) effects being significant for all the traits studied, indicating the involvement of additive as well as non-additive gene actions. However, the predominance of non-additive gene action was evident from higher SCA variances and GCA/SCA ratios less than unity for all traits. Among the parents, IS-4634, SLB-124, Solapur Dagadi, and Barshi Jute were identified as superior general combiners for grain yield and associated traits, while ICSB-450B was a good combiner for earliness. The estimation of SCA effects and heterosis revealed several superior hybrid combinations, among which SLB-12 × ICSB-450B exhibited the highest SCA effect and heterosis for grain yield per plant, whereas PMS-20B × ICSB-29B showed exceptional performance for stover yield. Crosses such as SLR-31 × AKMS-66-2B and SLR-31 × ICSB-450B also demonstrated consistent superiority across multiple traits. The high magnitude of heterosis and SCA effects observed in these crosses highlights the importance of dominance and epistatic interactions in governing yield traits. The study further facilitated the identification of promising heterotic patterns, which can be effectively utilized in hybrid breeding programs. Overall, the results suggest that exploitation of heterosis would be the most effective approach for improving grain and biomass yield in sorghum, while the identified superior parents can be used in population improvement strategies.

Research Article | Published online : 27-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 237–245
Urvashi Mishra1, Komal Parmar2 and Poripurna Goswami*3
1-3 Department of Family and Community Resource Management, Faculty of Family and Community Science, The Maharaja Sayajirao University of Baroda, Vadodara - 390 002, Gujarat, India
Abstract
Indoor plants have garnered increasing attention for their diverse benefits, including enhancement of indoor air quality and promotion of psychological well-being. This study aimed to assess the level of awareness among homemakers regarding the health, environmental, and psychological benefits of indoor plants. A descriptive research design was employed, with purposive sampling of homemakers in Vadodara city. Data were collected through a structured questionnaire covering demographic characteristics, knowledge of indoor plants, and perceptions of their benefits. Analytical tools such as frequency distribution, percentage analysis, and Chi-square tests were used to examine associations between demographic variables and awareness levels. The findings indicated that most respondents demonstrated substantial awareness of aesthetic and psychological benefits, including stress reduction and mood enhancement. However, awareness of health-related advantages, such as air purification and mitigation of respiratory issues, was comparatively moderate. Significant associations were observed between awareness levels and variables such as education, age, and exposure to information sources like social media and gardening platforms. The results highlight a knowledge gap in health and environmental aspects, emphasizing the need for targeted educational interventions. Community-based programs and awareness campaigns can play a crucial role in promoting informed practices, thereby enhancing indoor environmental quality and supporting sustainable living.

Research Article | Published online : 29-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 246–251
Vishal Verma1, Rishabh Yadav*2, Roshani Chaurasiya3, Gyanendra Singh1, Divya Katiyar1 and Arjun Prasad Verma4
1 Department of Agricultural Extension, College of Agriculture, Banda University of Agriculture and Technology, Banda - 210 001, Uttar Pradesh, India 2 Department of Agricultural Extension, College of Agriculture, Banda University of Agriculture and Technology, Banda - 210 001, Uttar Pradesh, India 3 Department of Extension Education, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur - 848 125, Bihar, India 4 Department of Extension Education, IAS, Banaras Hindu University, Varanasi - 221 005, Uttar Pradesh, India
Abstract
The extent of digital technology utilisation has transformed information access and learning behaviour among postgraduate students, making internet use an important part of academic activities in State Agricultural Universities (SAUs). The study was conducted during 2024-25 in four SAUs of Uttar Pradesh. A total of 240 postgraduate students were selected through simple random sampling. Data were collected using a structured and pre-tested interview schedule and analysed using descriptive and inferential statistics. The findings indicated that 37.50 per cent of students used the internet for 2-4 hours and an equal proportion for 5-7 hours daily, while 10.80 percent reported usage exceeding 10 hours. Weekly usage showed that 44.20 per cent of respondents spent 20-30 hours online, and 33.30 per cent spent more than 30 hours online. Academic purposes, such as searching for general information, research work, and accessing research papers, have the highest internet use, whereas non-academic activities like entertainment and social networking generally have usage of less than one hour. Internet usage among postgraduate students is largely academic-oriented; however, the presence of high-frequency users highlights the need for promoting efficient and responsible use through digital literacy and time management interventions.

Research Article | Published online : 30-Apr-2026

Research Journal of Agricultural Sciences
Vol : 17 - Issue : 2 ; 252–255
B. V. G. Prasad*1 and S. Chakravorty2
1 Assistant Professor (Horticulture), College of Horticulture, Parvathipuram, Dr. Y. S. R. Horticultural University, Andhra Pradesh - 535 502, India 2 Professor (Horticulture), Department of Horticulture and Post-Harvest Technology, Institute of Agriculture, Visva-Bharati (A Central University), West-Bengal - 731 236, India
Abstract
The Field experiment was conducted to find out the response of mulches and micronutrients on growth and yield of broccoli with an objective to estimate production economics. The experiment was laid out in split-plot design with three main plots (mulches), nine sub plots (micronutrients) and twenty-seven interaction treatments (mulches x micro nutrients) and each treatment was replicated thrice. The results revealed that mulches and micronutrients as well as their interaction significantly affected sprout production and yield of broccoli. Remarkable increase in sprout number (5.59), maximum sprout weight (62.89g) and highest sprout yield (5.59 t/ha) were observed where plants were grown under paddy straw mulch @7 t. ha-1 and treated with Zn-1% + Bo1-0.5% (M1mn6).

Research Article | Published online : 30-Apr-2026