|Mulberry breeders encounter the problem of how to choose suitable breeding material and moreover, there is little purpose in assembling the material unless it is effectively used and preserved. Therefore, a need was felt to develop a catalogue on evaluation of gene pool comprising 58 genotypes involving 33 and 25 genotypes of temperate and tropical origin. The 58 genotypes involved five species of mulberry namely Morus alba, Morus indica, Morus maulticaulis, Morus bombycis, and Morus Kayayama. Visual observation namely growth and branching nature, young and matured shoot colour, phyllotaxy, lenticel density and shape, bud shape, leaf apex, shape, margin, base, surface, texture, nature, lobation, colour, glossiness were recorded. The visual observations can be of immense help in selection of mulberry genotype through breeding procedure suited as a feed to silkworm. In the present investigation performance of M. multicaulis was found to be superior in most of the metric traits except in root ability. Maximum root ability was recorded in M. alba. Comparative evaluation of M. alba and M. indica revealed the superiority of former in most of the economic traits except in internodal distance. A comparison on the pooled data of 58 genotypes revealed the superiority of temperate mulberry genotypes in most of the characters studied except root ability. Comparative analysis of 5 species of mulberry on incidence of common diseases and pests namely powdery mildew, leaf spot and margaronia, belonging to temperate regions revealed percentage disease index of 9.84, 30.09, 3.67 and 15.77, 28.09, 3.49 in tropical genotypes respectively. The information generated indicates that tropical genotypes are more susceptible to powdery mildew when grown under temperate ecosystem. Behaviour of 5 species of mulberry indicated superiority of M. multicaulis with regard to incidence of diseases and pests. M. bombycis in general has shown the greatest cold hardiness and resistance to die-back which is of prime importance for taking up crop improvement studies under temperate ecosystem. Sexuality of 58 genotypes belonging to 5 species of mulberry from diverse origin revealed 72.70, and 76.00 are dioecious, in temperate and tropical genotypes, respectively. The appearance of bisexual flowers was found to be occasional.