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Volume - 2 - January-March 2011
Research Journal of Agricultural Sciences Vol : 2 - Issue : 1 ; 01-07
S I El-Sherif, H M Rashad, M H Belal, Y M El-Sebay* and N A Abd El-Latif*
Faculty of Agriculture, Cairo University, Giza, Egypt *Plant Protection Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
The horizontal and vertical movements of chloropyrifos (Dursban 48%) and its biological activity on the workers of the two subterranean termites Anacanthotermes ochraceus (Fam. Hodotermitidae) and Psammotermes hybostoma (Fam. Rhinotermitidae) was investigated in a sand loamy soil at El-Fayoum Governorate, Egypt. Cylindrical holes (30cm in diameter, 30cm deep and 3m apart) were treated with chloropyrifos at the recommended dose (1% a.i. aqueous emulsion at a rate of 4 liters/ hole). Soil samples were taken from the treated holes at different horizontal and vertical distances from treatment locations at 1, 7, 30, 90, 180, 270 and 360 days after treatment and the mortalities of the workers of the two considered termite species determined after 3 days of exposure to treated soil. The residues of chloropyrifos at the different tested horizontal and vertical distances combinations at the different sampling dates were also determined. Chloropyrifos penetrated into the soil horizontally to distances of at least 60cm from treatment locations from all directions, vertically upwards to distances of at least 10cm above treatment locations and vertically downwards to at least 60cm below them from all directions. Chloropyrifos persisted in the treated soil for at least 360 days with a maximum residue of about 400ug/ g at treatment location 1 day after treatment and a minimum residue of about 236ug/ g at the same location 360 days after treatment. The efficacy of chloropyrifos decreased with its penetration into the soil either horizontally or vertically along with time. Chloropyrifos remained considerably effective against the workers of both A. ochraceus and P. hybostoma as long as its residue in soil recorded about 2ug/ g or more. The horizontal movement of chloropyrifos in soil indicated that the recommended horizontal distance between any two holes for an effective control of termite workers in a sand loamy soil is 60cm from all directions.
Research Journal of Agricultural Sciences Vol : 2 - Issue : 1 ; 08-12
Vishwanath K, Prasanna K P R, Pallvi H M, Rajendra Prasad S, Ramegowda, Devaraju P J, *Ananthararayanan T V
Department of Seed Science and Technology, University of Agricultural Sciences, GKVK, Bangalore-65, Karnataka, India *Division of Plant Genetic Recourses, Indian Institute of Horticultural Research, Hesaraghatta, Bangalore, Karnataka, India
Abstract
Varietal development and its identification is one of the most important aspects of seed industry and seed trade. Due to continuous breeding programme by using elite lines, it has become difficult to identify and characterize these varieties on the basis of morphological characters alone. This has led to the exploration of new stable characters including genetic makeup to be used as markers for varietal identification. The present study includes the identification of different varieties (24) of tomato on the basis of their protein profile. Protein was extracted from sprouted seeds using electrophoresied on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). After fixation and coomaasie blue staining, all the varieties were identified according to their differences in the banding pattern and staining intensities.


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0976-1675
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2249-4538
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