|The present study was conducted in outdoor cemented ponds comprised of three factors i.e. two supplementary feeds (D1, routine diet vs. D2, diet supplemented with vitamin-mineral mixture @ 2%), two feeding rates (R1, 3% BW vs. R2 4% BW) and two feeding frequencies (F1, once-a-day vs. F2, twice-a-day feeding). Thus, there were 8 treatments combinations viz. T1: (D1, R1, F1), T2: (D1, R1, F2), T3: (D1, R2, F1), T4: (D1, R2, F2), T5: (D2, R1, F1), T6: (D2, R1, F2), T7: (D2, R2, F1) and T8: (D2, R2, F2). The diets were fed for a period of 8 months and the effect of different treatments on biochemical composition of flesh in terms of total proteins, total lipids, total carbohydrate, ash and moisture of fish from each treatment was analyzed during the pre-spawning (July) and post-spawning/resorption (September) period. During pre-spawning period, flesh protein content was maximum in T8 (12.78%) and minimum in T7 (11.71%), total lipid was maximum in T6 (1.53) and minimum in T5 (1.15), total carbohydrate was maximum in T8 (2.58%) and minimum in T1 (2.13%), moisture content was maximum in T8 (80.49%) and minimum in T1 (77.76%) and ash content was maximum in T1 (1.77%) and minimum in T8 (1.33%) and the difference among all the treatments were significant. During post-spawning/resorption period, the flesh protein content was maximum in T8 (13.10%) and minimum in T7 (12.12%), total lipid was maximum in T6 (1.81%) and minimum in T5 (1.35%), total carbohydrate was maximum in T4 (2.86%) and minimum in T7 (2.38%), moisture content was maximum in T7 (79.55%) and minimum in T1 (77.25%) and ash content was maximum in T1 (1.64%) and minimum in T8 (1.37%) and the difference among all treatments were significant. Not much difference was recorded in different parameters during pre-spawning and post-spawning period. In contrast, total carbohydrate content in fish increased with increase in feeding rate, feeding frequency as well as supplementation of vitamin mineral mixture in the diet. Similar trends were related in case of moisture content, while ash content decreased in fish fed twice with vitamin-mineral supplemented diet at both the feeding rates (3% and 4% BW). Overall results of vitamin-mineral supplementation of diets and feeding regimes was not found to have any positive effect on the flesh quality of fish.