Research Journal of Agricultural Sciences
Vol : 10 - Issue : 4 ; 659-667
B Suma*, R Sujatha and R S Maheswari
Department of Plantation Crops and Spices,
College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur - 680 656, Kerala
The study was undertaken to assess the genetic diversity among long pepper genotypes using morphological and random amplified polymorphic DNA (RAPD) markers. The research work was performed during 2010 to 2013 at Department of Plantation Crops and Spices, and Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur, Kerala. Among the forty two accessions studied for morphological traits, it was noticed that thirty eight accessions were female, three male and one non-flowering type revealed wide variation among accessions in the characteristics like growth habit, leaf lamina and base shape, leaf margin, spike shape, texture, colour etc. 56 genotypes were screened with four selected random decamer primers (RY15, OPA6, RN8 and RN12). RAPD analysis yielded thirty eight bands with 94.7 percent polymorphism among the genotypes. The mean polymorphic information content (PIC) indicated that the marker system is effective in detecting polymorphism. Genetic distances were computed using Dice coefficient and the similarity coefficient ranged from 0.02 to 0.79. Based on genetic distance, UPGMA clustering (unweighted pair-group method with arithmetic means) gave a dendrogram with three major clades. Collections originating from various parts of the country did not form well defined distinct groups and were interspersed with each other indicating no association between RAPD pattern and geographic origin of genotypes.
| Published online : 19-Dec-2019