|1,3,4Department of Microbiology,
Dinabandhu Andrewas College, Deparrshnabghata, 24 Parganas (South), Kolkata - 700 084, West Bengal, India
2Department of Microbiology, Rabindra Mahavidyalaya, Champadanga, Hooghly, W.B. 712401
|This study deals with the isolation of fungal communities and comparison seed borne mycoflora between Coastal zone seeds (Digha; West Bengal) and Indo-gangetic areas (Burdwan, Hooghly, Howrah; West Bengal) and to identify and classify them by standard blotter method and agar plate technique. Coastal areas seed samples were collected from the sea side of Bay of Bengal, Digha, West Bengal and the Indo-gangetic areas seed samples were collected from Burdwan, Hooghly, Howrah; West Bengal. The seeds were stored in sterile screw cap bottles for further analysis. Phenotypic and genotypic characterization of fungal isolates were done using above tow methods. Six fungal genera including Aspergillus sp., Penicillium sp., Rhizopus sp., Mucor sp., Alternaria sp., Macrophomina sp. Of them Aspergillus sp. and Alternaria sp. are the most frequent members. It was found that the rate of germination of coastal zone seeds were more than the rate of germination of Indo-gangetic zones seeds. Whereas the fungal community of coastal areas were appeared more frequent and destructive than indo-gangetic region. The germination percentage of oil seeds of indo-gangetic areas appeared respectively 92% and 96% where the germination percentage of oil seeds of coastal region appeared respectively 64% and 72%, and the germination percentage of cereal crops of indo-gangetic areas (Pratiksha, Swarna, Khas, Minikit, Kalma) appeared respectively 88%, 92%, 84%, 96%, 80%. Whereas the germination percentage of all those cereal crops (Protiksha, Swarna, Khas, Minikit, Kalma) of coastal region appeared respectively 60%, 68%, 56%, 72% and 52%. In the other hand the appearance of fungal community were more frequent of coastal areas than indo-gangetic region. The percentage of fungal growth of oil seed of indo-gangetic region (oil seeds) were respectively 35% and 20%. Whereas in coastal areas it was respectively 50% and 45%. The fungal growth of different cereal crops (Protiksha, Swarna, Khas, Minikit, Kalma) of indo-gangetic and coastal areas were respectively (20%, 18%, 25%, 15%, 28%) and (38%, 35%, 40%, 30%, 44%).