P- ISSN: 0976-1675
E- ISSN: 2249-4538

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Antibacterial Activity and Phytochemical Analysis of Biosynthesized Copper Nanoparticles Produced by Nyctanthes arbortristis Leaves Extract

Nyctanthes arbortristis, also known as Night jasmine or Parijaat, is a most common medicinal plant in India and its neighbouring countries. In this work, Nyctanthes leaves extract was used in the green synthesized copper nanoparticles. An examination of the Nyctanthes leaves extract's phytochemistry reveals the presence of substances including steroids, flavonoids, and polyphenols, tannins, proteins and carbohydrate compound. The secondary metabolites were confirmed by specific test. Scanning electron microscopy SEM, EDX, XRD, TEM and UV-Spectra were used to analyze the biosynthesized copper nanoparticles. Against the gram-negative Pseudomonas aeruginosa bacteria, biosynthesized copper nanoparticles demonstrated antibacterial properties. The results of that study show greater value of zone inhibition was found to be 12 mm at 200 µg/mL of CuNPs concentration of Nyctanthes arbortristis. The study concluded that the Nyctanthes arbortristis plant leaves extract produced copper nanoparticles that have antibacterial activity. Antibacterial sensitivity was proof against Pseudomonas bacteria. Preliminary phytochemical studies show the presence of secondary metabolites such as alkaloids, Protein, Carbohydrates, phenols and terpenoids.

Research Article | Published online : 30-Nov-2023