Research Journal of Agricultural Sciences
Vol : 15 - Issue : 15 ; 1105–1113
C. Initha Lebanon Ebency*, M. Flory Shobana1, S. Tharani2, Sharmy Saimon Mano3 and N. Vanitha4
* Department of Microbiology, Annai Violet Arts and Science College, Chennai - 600 053, Tamil Nadu, India
1, 3 Department of Biotechnology, Hindusthan College of Arts and Science, Avinashi Road, Behind Nava India, Coimbatore - 641 028, Tamil Nadu, India2
2 GRG BIRAC E- YUVA Centre, PSGR Krishnammal College for Women, Coimbatore - 641 004, Tamil Nadu, India
4 Department of Microbiology, Hindusthan College of Arts and Science, Avinashi Road, Behind Nava India, Coimbatore - 641 028, Tamil Nadu, India
Abstract
Actinomycetes, Gram-positive, filamentous bacteria, thrive in diverse environments and are prolific antibiotic producers. Streptomyces species, a key genus, produce around 80% of recognized secondary metabolites, including glycopeptides, beta-lactams, and aminoglycosides. This study aimed to produce and characterize bioactive compounds from Actinomycetes and screen their antioxidant properties. Biochemical tests confirmed the isolate's properties, showing positive results for starch, casein, gelatin, and cellulase hydrolysis, urea hydrolysis, nitrate reduction, catalase, H2S production, indole, MR, Voges-Proskauer, and citrate tests, indicating diverse enzymatic and metabolic capabilities. The bioactivity of isolates was confirmed against P. aureus, E. coli, Salmonella, and Proteus, with significant inhibition zones. The crude compound was purified via thin layer chromatography (TLC) with a retention factor of 0.83 and exhibited antimicrobial activity. The isolate was identified as Streptomyces longisporoflavus through 16s rRNA analysis.
C. Initha Lebanon Ebency*, M. Flory Shobana1, S. Tharani2, Sharmy Saimon Mano3 and N. Vanitha4
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Research Article | Published online : 03-Sep-2024